Effect of whey and cassava starch on the production of honey powder: Spectroscopic and morphological analysis
DOI:
https://doi.org/10.58951/fstoday.2026.001Keywords:
Honey, Whey, Cassava starch, FT-IR, DLS, SEMAbstract
Honey is considered a nutritious food, produced primarily by bees (Apis mellifera L.) from flower nectar. However, its high viscosity and handling difficulties have driven the development of honey powder production. The use of adjuvants such as whey and cassava starch, due to their versatility and availability, is a viable option for honey powder production. Therefore, mixtures of honey, whey, and cassava starch were evaluated. The mixtures were dried in a forced air oven at 60 °C for 24 h. The resulting powders were physicochemically characterized and analyzed by FT-IR, DLS, and SEM. The mixture with the highest whey content showed a tendency to slightly decrease the absorption peaks in the spectral range of 1400 to 700 cm−1 in FT-IR spectral analysis. The electrostatic stability showed values of −34.7907 mV, a peak distribution of −35.3467 mV with a conductivity of 0.0091 mS/cm and an electrophoretic mobility of −2.7116 µm*cm/Vs. The morphology of the resulting powder consisted of agglomerated particles with starch granules ranging in size from approximately 45.7 to 90.2 µm. Whey and cassava starch are used as additives and stabilizing agents to produce powdered honey.
References
Allen, K. E., Carpenter, C. E., & Walsh, M. K. (2007). Influence of protein level and starch type on an extrusion‐expanded whey product. International Journal of Food Science & Technology, 42(8), 953–960. https://doi.org/10.1111/j.1365-2621.2006.01316.x
AOAC. (2023). Official methods of analysis of AOAC International (22nd ed.). AOAC International.
Baglio, E. (2018). Honey: Processing Techniques and Treatments (pp. 15–22). https://doi.org/10.1007/978-3-319-65751-6_2
Bravo-Núñez, Á., Garzón, R., Rosell, C. M., & Gómez, M. (2019). Evaluation of starch–protein interactions as a function of pH. Foods, 8(5), 155. https://doi.org/10.3390/foods8050155
Breuninger, W. F., Piyachomkwan, K., & Sriroth, K. (2009). Tapioca/cassava starch. In Starch (pp. 541–568). Elsevier. https://doi.org/10.1016/B978-0-12-746275-2.00012-4
Britchenko, I., & Mickiewicz, B. (2022). Main trends and development forecast of bread and bakery products market. VUZF Review, 7(3), 113–123. https://doi.org/http://dx.doi.org/10.2139/ssrn.4235605
Chandrakar, N., Padhi, S., Saraugi, S. S., Sehrawat, R., Singh, A., & Routray, W. (2024). Whey protein and maltodextrin conjugated foam-mat dried honey powder: Functional, physicochemical, structural, rheological and thermal characterization. Chemical Engineering Research and Design, 209, 367–379. https://doi.org/10.1016/j.cherd.2024.08.015
Chandrapala, J., Duke, M. C., Gray, S. R., Zisu, B., Weeks, M., Palmer, M., & Vasiljevic, T. (2015). Properties of acid whey as a function of pH and temperature. Journal of Dairy Science, 98(7), 4352–4363. https://doi.org/10.3168/jds.2015-9435
Chaven, S. (2023). Confectionery and Bakery Products; Including Honey. In Food Safety Management (pp. 291–303). Elsevier. https://doi.org/10.1016/B978-0-12-820013-1.00045-0
Dodds, J. (2024). Techniques to analyse particle size of food powders. In Handbook of Food Powders (pp. 249–268). Elsevier. https://doi.org/10.1016/B978-0-323-98820-9.00070-3
Filomeni, G., Graziani, I., De Zio, D., Dini, L., Centonze, D., Rotilio, G., & Ciriolo, M. R. (2012). Neuroprotection of kaempferol by autophagy in models of rotenone-mediated acute toxicity: possible implications for Parkinson’s disease. Neurobiology of Aging, 33(4), 767–785. https://doi.org/10.1016/j.neurobiolaging.2010.05.021
Hazlett, R., Schmidmeier, C., & O’Mahony, J. A. (2021). Approaches for improving the flowability of high-protein dairy powders post spray drying – A review. Powder Technology, 388, 26–40. https://doi.org/10.1016/j.powtec.2021.03.021
He, R., Du, J., Feng, A., Zhang, J., Zhong, G., Fu, N., & Yang, T. (2025). Physical modification of cassava starch by alternating magnetic field treatment: Effects on fine structure, retrogradation and in vitro digestibility. Food Chemistry, 471, 142650. https://doi.org/10.1016/j.foodchem.2024.142650
Ke, C., & Li, L. (2023). Influence mechanism of polysaccharides induced Maillard reaction on plant proteins structure and functional properties: A review. Carbohydrate Polymers, 302, 120430. https://doi.org/10.1016/j.carbpol.2022.120430
Masyithoh, D., Jaya, F., Estiasih, T., Ira Febriani, S., Claudhia Falaq, E., & Kurniawan, F. (2025). Characterization of vacuum-dried honey powder: effect of honey type and carrier composition on functional, physicochemical, and antioxidant properties. International Journal of Food Properties, 28(1). https://doi.org/10.1080/10942912.2025.2557992
Montes, C., Villaseñor, M. J., & Ríos, Á. (2019). Analytical control of nanodelivery lipid-based systems for encapsulation of nutraceuticals: Achievements and challenges. Trends in Food Science & Technology, 90, 47–62. https://doi.org/10.1016/j.tifs.2019.06.001
Nabgan, M., Shariatifar, N., Jahed-Khaniki, G., Molaee-Aghaee, E., & Sani, M. A. (2025). Development and characterization of whey protein packaging film loaded with Pickering emulsion stabilized marjoram (Origanum majorana L.) essential oil. International Journal of Biological Macromolecules, 334, 149178. https://doi.org/10.1016/j.ijbiomac.2025.149178
Pelegrine, D. H. G., & Gasparetto, C. A. (2005). Whey proteins solubility as function of temperature and pH. LWT - Food Science and Technology, 38(1), 77–80. https://doi.org/10.1016/j.lwt.2004.03.013
Saji, R., Ramani, A., Gandhi, K., Seth, R., & Sharma, R. (2024). Application of FTIR spectroscopy in dairy products: A systematic review. Food and Humanity, 2, 100239. https://doi.org/10.1016/j.foohum.2024.100239
Samborska, K. (2019). Powdered honey – drying methods and parameters, types of carriers and drying aids, physicochemical properties and storage stability. Trends in Food Science & Technology, 88, 133–142. https://doi.org/10.1016/j.tifs.2019.03.019
Samborska, K., Barańska-Dołomisiewicz, A., Jedlińska, A., Costa, R., Klimantakis, K., Mourtzinos, I., & Nowacka, M. (2025). Valorization of Spent Osmotic Solutions by Production of Powders by Spray Drying. Applied Sciences, 15(12), 6927. https://doi.org/10.3390/app15126927
Santos, A. C. dos, Biluca, F. C., Braghini, F., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2021). Phenolic composition and biological activities of stingless bee honey: An overview based on its aglycone and glycoside compounds. Food Research International, 147, 110553. https://doi.org/10.1016/j.foodres.2021.110553
Saraugi, S. S., Chandrakar, N., Padhi, S., & Routray, W. (2026). Insights into honey powder production: Key factors, production methods, physicochemical characterization, and applications. Food Chemistry, 500, 147402. https://doi.org/10.1016/j.foodchem.2025.147402
Sathivel, S. (2013). Application of honey powder in bread and its effect on bread characteristics. Journal of Food Processing & Technology, 04(11). https://doi.org/10.4172/2157-7110.1000279
Setiowati, A. D., Wijaya, W., & Van der Meeren, P. (2020). Whey protein-polysaccharide conjugates obtained via dry heat treatment to improve the heat stability of whey protein stabilized emulsions. Trends in Food Science & Technology, 98, 150–161. https://doi.org/10.1016/j.tifs.2020.02.011
Simunkova, H., Pessenda-Garcia, P., Wosik, J., Angerer, P., Kronberger, H., & Nauer, G. E. (2009). The fundamentals of nano- and submicro-scaled ceramic particles incorporation into electrodeposited nickel layers: Zeta potential measurements. Surface and Coatings Technology, 203(13), 1806–1814. https://doi.org/10.1016/j.surfcoat.2008.12.031
Soler, A., Dominguez-Ayala, J. E., Morales-Sánchez, E., Cabrera-Ramírez, Á. H., Sifuentes-Nieves, I., Velazquez, G., & Mendez-Montealvo, G. (2026). Unraveling the crystalline and double helical characteristics and their impact on the functionality of dry-heated cassava and corn starch. International Journal of Biological Macromolecules, 340, 150004. https://doi.org/10.1016/j.ijbiomac.2025.150004
Suhag, Y., & Nanda, V. (2016). Optimization for spray drying process parameters of nutritionally rich honey powder using response surface methodology. Cogent Food & Agriculture, 2(1). https://doi.org/10.1080/23311932.2016.1176631
Tenore, G. C., Ritieni, A., Campiglia, P., & Novellino, E. (2012). Nutraceutical potential of monofloral honeys produced by the Sicilian black honeybees (Apis mellifera ssp. sicula). Food and Chemical Toxicology, 50(6), 1955–1961. https://doi.org/10.1016/j.fct.2012.03.067
Tomczyk, M., Zaguła, G., Tarapatskyy, M., Kačániová, M., & Dżugan, M. (2021). The effect of honey variety on the quality of honey powder. Journal of Microbiology, Biotechnology and Food Sciences, 9(5), 949–954. https://doi.org/10.15414/jmbfs.2020.9.5.949-954
Toniazzo, T., Collado-González, M., Tadini, C. C., & Mackie, A. R. (2023). Evaluation of physicochemical properties of honey powder using rice and pea proteins as carriers. Food Research International, 167, 112692. https://doi.org/10.1016/j.foodres.2023.112692
Torley, P. J., Rutgers, R. P. G., D’Arcy, B., & Bhandari, B. R. (2004). Effect of honey types and concentration on starch gelatinization. LWT - Food Science and Technology, 37(2), 161–170. https://doi.org/10.1016/j.lwt.2003.08.002
Verma, D. K., Patel, A. R., Tripathy, S., Gupta, A. K., Singh, S., Shah, N., Utama, G. L., Chávez-González, M. L., Zongo, K., Banwo, K., Niamah, A. K., & Aguilar, C. N. (2024). Processing and formulation technology of nutritional and functional food products by utilizing cheese and/or paneer whey: A critical review. Journal of King Saud University - Science, 36(11), 103508. https://doi.org/10.1016/j.jksus.2024.103508
Vosoghi, M., Yousefi, S., & Honarvar, M. (2025). Physicochemical and sensory properties of honey powder from different climatic regions. Applied Food Research, 5(1), 100843. https://doi.org/10.1016/j.afres.2025.100843
Wang, Z., Mhaske, P., Farahnaky, A., Kasapis, S., & Majzoobi, M. (2022). Cassava starch: Chemical modification and its impact on functional properties and digestibility, a review. Food Hydrocolloids, 129, 107542. https://doi.org/10.1016/j.foodhyd.2022.107542
Wu, Y., & Guo, Q. (2025). Effect of whey protein on the formation, structure and gastrointestinal breakdown of quinoa flour-based composite gels. Food Hydrocolloids, 160, 110775. https://doi.org/10.1016/j.foodhyd.2024.110775
Yadav, R., Aralkar, S., Prasad, K. V., Firake, D. M., Aradwad, P., Raju, D. V. S., & Kv, S. (2026). Trends in honey spray drying: A comprehensive analysis of drying conditions, process optimization, physicochemical properties, honey powder enrichment and industrial applications. Trends in Food Science & Technology, 168, 105508. https://doi.org/10.1016/j.tifs.2025.105508
Yamul, D. K., & Lupano, C. E. (2005). Whey protein concentrate gels with honey and wheat flour. Food Research International, 38(5), 511–522. https://doi.org/10.1016/j.foodres.2004.11.005
Zhang, J., Liu, Y., Wang, P., Zhao, Y., Zhu, Y., & Xiao, X. (2025). The Effect of Protein–Starch Interaction on the Structure and Properties of Starch, and Its Application in Flour Products. Foods, 14(5), 778. https://doi.org/10.3390/foods14050778
Zhang, X., Wang, Q., Liu, Z., Zhi, L., Jiao, B., Hu, H., Ma, X., Agyei, D., & Shi, A. (2023). Plant protein-based emulsifiers: Mechanisms, techniques for emulsification enhancement and applications. Food Hydrocolloids, 144, 109008. https://doi.org/10.1016/j.foodhyd.2023.109008
Zhen, Y., Wang, K., Wang, J., Qiao, D., Zhao, S., Lin, Q., & Zhang, B. (2022). Increasing the pH value during thermal processing suppresses the starch digestion of the resulting starch-protein-lipid complexes. Carbohydrate Polymers, 278, 118931. https://doi.org/10.1016/j.carbpol.2021.118931
Zhu, F. (2015). Interactions between starch and phenolic compound. Trends in Food Science & Technology, 43(2), 129–143. https://doi.org/10.1016/j.tifs.2015.02.003
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Copyright (c) 2026 Ulin Antobelli Basilio-Cortes, Daniel González-Mendoza, Dagoberto Durán-Hernández, Alexis Alejandro Salazar-Navarro, Benjamín Valdez-Salas, Ernesto Beltrán-Partida, José Gregorio Joya-Dávila, Lourdes González-Salitre

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